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Prevention of Cross-Contamination
To minimize the risk of cross-contamination and to prevent contact with potentially infectious materials,
always complete the processes involving sample preparation such pipetting of blood samples in a designated
part of the benchtop or safety hood before starting PCR setup.
To further minimize the risk of contamination, always carry out sample preparation, PCR setup, and the PCR
run itself in separate rooms specially designated for each phase in the workflow.
Safety Information Laboratory Procedures
Handle all samples as potentially infectious, using safe laboratory procedures. As the sensitivity and titer of
potential pathogens in the sample material varies, the operator must optimize pathogen inactivation by the
Lysis/Binding Buffer or take appropriate measures, according to local safety regulations.
RBC Bioscience does not warrant that samples treated with Lysis Buffer are completely inactivated and
noninfectious. After sample processing is completed, remove and autoclave all disposable plastics.
• Do not eat, drink or smoke in the laboratory work area.
• Wear protective disposable gloves, laboratory coats, and eye protection when handling samples and kit
reagents.
• Do not contaminate the reagents with bacteria, viruses, or nucleases. Use disposable pipettes and nuclease-
free pipette tips only to remove aliquots from reagent bottles. Use the general precautions described in the
literature.
• Wash hands thoroughly after handling samples and reagents.
• Complete each phase of the PCR/RT-PCR workflow before proceeding to the next phase. For example, you
should finish PCR/RT-PCR sample preparation before starting PCR/RT-PCR setup. Sample preparation, PCR/
RT-PCR setup and the PCR/RT-PCR run itself should also be performed in separate locations.
Waste Handling
• Discard unused reagents and waste in accordance with country, federal, state, and local regulations.
• Safety Data Sheets (SDS) are available upon request from the local distributor.
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