MM138-003 – Operator Manual
59
1) Clean plate and ring, using ethanol moistened paper, just before to use them.
1) Place the high ring on the top of the cold plate and insert the sample inside it. Maintain the sample in
the needed position with the tweezers.
2) Fill the ring hole with MCC (Milestone Cryoembedding compound) up to 2mm under the edge.
3) Place the heat extractor over the ring and close the PrestoCHILL cover. Wait the freezing time and
then check the freezing completion lifting the heat extractor.
The freezing time depends on the dimension and the sample type, it is :
- 120 seconds for RNG7028-25 at -80°C
- 180 seconds for RNG7037-25 at -80°C
4) At the end of the freezing time, add MCC to completely fil the ring hole and place on it the chuck and
the heat extractor. Freeze for 20 seconds in order to attach the block to the chuck.
5) Open the unit and remove the heat extractor with the frozen block attached.
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