ImageXpress Micro High Content Imaging System Options User Guide
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Before you run an experiment:
• Allow the instrument to warm up for at least two hours.
• Make sure the plate sealing ring is at 37°C before use, either by
keeping it inside the instrument or inside an incubator. Incorrect
sealing-ring temperature can cause temperature fluctuation.
• Wait 30 minutes to 1 hour for the plate to reach equilibrium. You
can use a journal to set this waiting period.
The current temperature in the chamber is displayed on the
front of the controller.
• If there is interstitial space between wells, pipette deionized
water or media into these areas. This helps to increase the
thermal mass of the plate and reduces overall evaporation.
1.
Note: Due to the changes in Z height over time, you might need
to adjust the plate focus parameters in the MetaXpress software
occasionally. To do so, open the MetaXpress software, and then
click Screening > Plate Acquisition Setup > Plate tab > Edit Plate
Bottom Settings. Increase the adjacent well max variation to
accommodate the added Z-height variation introduced by the
fluctuation of temperature.
When setting laser autofocus options, make sure that the
instrument and plate temperatures are at equilibrium. Room-
temperature laser autofocus settings are not ideal when the
system is used at higher temperatures.
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