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2
Fluidics Option Operations
The ImageXpress
®
Fluidics option consists of a single-channel, fluidics
robot. This robot picks up tips from a tip box and draws fluid from and
delivers fluid to a given well on the compound and sample plates. For the
sample plate, the solenoid-operated plate shutter opens briefly, allowing
the robot access to a given well without compromising the
environmental control.
The robot is capable of dispensing volumes between 3 μL and 200 μL
with an accuracy of ±5% or ±1 μL.
The ImageXpress Fluidics option must be installed with the Environment
Control option. It is not compatible with the Transmitted Light option.
See Also:
• Environment Control Option Operations on page 9.
This chapter contains the following sections:
• Fluidics Hardware on page 31
• Setting Up Fluidics Hardware on page 33
• Configuring Fluidics Software (Stations and Properties) on
page 35
• Doing Fluidics Experiments on page 43
• Preventing Evaporation from Compound Plates on page 49
Fluidics Hardware
The ImageXpress Fluidics option consists of the following hardware
components. See Figure 2-1.
• Single-channel pipettor. Used to transfer fluid between two
compound/media plates and the sample plate.
• Shutter. Allows the system to maintain the environment when
not actively pipetting.
• Plate heaters. Used to heat the compound plates. These heat a
little higher than the displayed temperature, which represents
the temperature of the liquid in the well.
• Tip adapters and tip rack positions. Compatible with FLIPR
®
Tetra
96 tips (Part 9000-0761, 50 racks/case) or 384 tips (Part 9000-
0763, 50 racks/case).
ImageXpressOptionsUserGuide.book Page 31 Wednesday, September 9, 2015 3:54 PM
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