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Molecular Devices ImageXpress Pico Calibration Kit Guide

Molecular Devices ImageXpress Pico
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ImageXpress Pico Automated Cell Imaging System Calibration Kit Guide
18 5063669 E
Adjusting an Objective Correction Collar
The 40x objective and 63x objective have application-optimized correction collars to
compensate for well bottom thickness or coverslip thickness. The collars have a range of
0mm to 2mm correction. Changing this setting adjusts the distances between components
inside the objective barrel. Image quality and resolution are very dependent on properly
setting these collars.
The settings to be used depend on the well bottom thickness of the plate or the coverslip
thickness on the slide on which the specimen is mounted. In general, set the correction collar
for the physical thickness of the plate or slide that you are imaging. The physical thickness
can be determined by the plate specifications from the plate manufacturer.
Note: Do not use a plate, slide, or coverslip with a thickness that is out of the range of
the correction collar for the selected objective.
Before adjusting an objective correction collar, review the following:
Access only the user-serviceable components inside the enclosure as described in the
procedure. Avoid contact with other components as they can be damaged or knocked
out of alignment.
To prevent dust from collecting inside the instrument, keep all access doors closed
unless you are performing maintenance tasks.
Ensure that all components and access doors are closed before starting the instrument.
CAUTION!
To prevent skin oils from damaging the optical coatings, Molecular Devices
recommends that you wear powder-free disposable gloves when handling
objectives and filter cubes.
With the instrument power on, do not manually rotate the objective turret.
Manually rotating the objective turret can damage the instrument.
You would typically adjust a correction collar as part of setting up an acquisition.

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Molecular Devices ImageXpress Pico Specifications

General IconGeneral
BrandMolecular Devices
ModelImageXpress Pico
CategoryLaboratory Equipment
LanguageEnglish

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