46
5
VARIOUS OBSERVATION METHODS
5.3.3. Settings for polarization observation
A
Illustration 033: A-B: Setting for polarization: Assembly of the polarizer
and analyzer.
All settings, including adjustment of
the lighting according to Koehler,
were made in bright field.
Place the polarizer (1) on the field dia-
phragm (2).
5
4
6
3
10x
B
Remove the rubber placeholder (3) on the
objective nosepiece (4) and insert the ana-
lyzer (5) in the slot.
The analyzer must engage securely.
Turn the 10x objective into working position.
Increase the illumination intensity slightly (6).
You will notice while looking into the micro-
scope with the polarizer and analyzer in
place and no specimen, that the field will
look dark.
Place the specimen on the stage and focus it
Adjust the field diaphragm until the image is
at least as large as field of view.
Contrast can be increased by reducing the
aperture diaphragm.
To Next Page
Loading...
