Chapter 8 Troubleshooting
2 Image Output to a PC Monitor
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Trouble Check point
The contrast is low.
• Check that the tone is adjusted correctly with the application software.
⇒ Refer to the instruction manual for the application software.
• Check that the black level is set appropriately with the application
software.
⇒ Refer to the instruction manual for the application software.
• Check that the size of the field diaphragm is adjusted so that it
circumscribes the view field.
• Check that the size of the aperture diaphragm is adjusted to
approximately 70% to 80% of the numerical aperture of the objective
lens.
• Check that an appropriate filter is used.
When using a phase contrast microscope or an interference microscope
(two luminous fluxes or multiple luminous fluxes), insert the specified
filter. With a black and white image, contrast is improved by inserting a
green interference filter (GIF). Contrast in color images is enhanced by
inserting a filter having a complementary color to that of the specimen.
• Check that the ambient light around the microscope does not affect.
If the ambient light affects, darken the room or attach a cap to the
binocular eyepiece.
• Check that the contrast of the specimen is sufficient.
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