What type of slide glass should I use to prevent fluorescence with Nikon Microscope?
- KKrista BurtonSep 12, 2025
If the slide glass is emitting fluorescence while using your Nikon Microscope, use a non-fluorescent glass slide.

What type of slide glass should I use to prevent fluorescence with Nikon Microscope?
If the slide glass is emitting fluorescence while using your Nikon Microscope, use a non-fluorescent glass slide.
How to put barrier filter in the optical path on Nikon ECLIPSE Si?
If the barrier filter is not in the optical path of your Nikon Microscope, push in the BA filter slider all the way, bringing the barrier filter into the optical path.
How to fix BA filter slider is not at the click position on Nikon ECLIPSE Si?
If the BA filter slider on your Nikon Microscope is not at the click position, push in the slider all the way and move the slider until it clicks.
How to fix EX filter slider is not at the click position on Nikon ECLIPSE Si?
If the EX filter slider on your Nikon Microscope is not at the click position, move the slider until it clicks.
What to do if the specimen's phase difference and the Ph objective type are incompatible on Nikon ECLIPSE Si Microscope?
If the specimen's phase difference and the Ph objective type are incompatible when using your Nikon Microscope, use an appropriate Ph objective for the specimen.
How to brighten diascopic illumination on Nikon ECLIPSE Si Microscope?
If the diascopic illumination of your Nikon Microscope isn't bright enough, try these steps: First, adjust the illumination by turning the brightness control knob almost to the maximum position. If that doesn't fully resolve the issue, widen the aperture diaphragm to allow more light to pass through.
How to fix Ph annular diaphragm of the phase contrast slider and the objective's phase ring image are not at matching positions on Nikon ECLIPSE Si Microscope?
If the Ph annular diaphragm of the phase contrast slider and the objective's phase ring image are not at matching positions on your Nikon Microscope, adjust the position of the annular diaphragm.
How to fix Ph annular diaphragm of the phase contrast slider and the Ph code of the objective are incompatible on Nikon ECLIPSE Si?
If the Ph annular diaphragm of the phase contrast slider and the Ph code of the objective are incompatible on your Nikon Microscope, bring a Ph annular diaphragm that has the same code as the objective's Ph code into the optical path.
How to fix phase contrast slider is not at the click position on Nikon ECLIPSE Si Microscope?
If the phase contrast slider on your Nikon Microscope is not at the click position, move the slider until it clicks.
What to do if specimen rises from stage surface on Nikon ECLIPSE Si?
If the specimen rises from the stage surface on your Nikon Microscope, stabilize it using the holder.
| Brand | Nikon |
|---|---|
| Model | ECLIPSE Si |
| Category | Microscope |
| Language | English |
Explains symbols on the product for user safety.
Defines WARNING and CAUTION symbols used in the manual.
Describes the basic observation method using diascopic illumination.
Explains the technique for observing unstained, colorless specimens.
Details the method for measuring polarization and refraction states.
Outlines the procedure for observing specimens stained with fluorescent dye.
Describes the technique for observing unstained specimens on a dark background.
Visual representations of how different parts connect for various configurations.
Step-by-step assembly for the primary bright-field microscopy setup.
Instructions for setting up the microscope for phase contrast observation.
Lists common issues related to image quality and appearance.
Covers operational difficulties such as focusing and image integration.
Addresses issues related to illumination and power supply.











