Dichroic Mirror and Filter Configurations of Fluorescence Mirror Units
Excitation
method
Mirror unit Dichroic mirror Excitation filter Barrier filter Applications
U
U-FUW
DM410
BP340-390
BA420IF
· Autofluorescence observation
· DAPI: DNA staining
· Hoechest 33258, 33342: Chromosome
U-FUN BP360-370
V U-FVN DM455 BP400-410 BA460IF
· Catecholamine observation
· Serotonin observation
· Tetracyline: Bones, teeth
BV U-FBVW DM455 BP400-440 BA460IF
· Quinacrine, quinacrine mustard:
Chromosome
· Thioflavine S: Lymphocyte
· Acriflavine: Nucleic acid
· ECFP
B
U-FBW
DM505
BP460-495
BA510IF
· FITC: Fluorescent antibody
· Acridine orange: DNA, RNA
· Auramine: Tubercle bacillus
· EGFP, S65T, RSGFP
U-FBN BP470-495
G U-FGW DM570 BP530-550 BA575IF
· Rhodamine, TRITC:Florescent antibody
· Propidium iodide: DNA
· RFP
Y U-FYW DM600 BP545-580 BA610IF Texas Red: Fluorescent antibody
Combinations for color separation
U U-FUNA DM410 BP360-370 BA420-460
For observing only the U-excitation stain
when using U-excitation stain together
with FITC.
B
U-FBWA
DM505
BP460-495
BA510-550
For observing only the B-excitation stain
when using B-excitation stain with TRITC
or Texas Red.
U-FBNA BP470-495
G
U-FGWA
DM570
BP530-550
BA575-625
For observing only the G-excitation stain
when using G-excitation stain together
with Cy5.
U-FGNA BP540-550
Exclusive combinations for fluorescent proteins
CFP U-FCFP DM455 BP425-445 BA460-510 For ECFP.
GFP U-FGFP DM490GFP BP460-480 BA495-540 For EGFP.
YFP U-FYFP DM515 BP470-500 BA515-560 For EYFP.
RFP U-FRFP DM565HQ BP535-555HQ BA570-625HQ For RFP.
mCherry U-FMCHE DM595 BP565-585 BA600-690 For mCherry.
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