Fig. 27
Fig. 28
Fig. 29
Fig. 30
0 Coarse and Fine Adjustment Knobs
1) Bring the objective as close as possible to the specimen without
touching and focus on the specimen roughly by means of the coarse
adjustment knobs 0, then fine focus with the fine adjustment
knobs 0. (Fig. 27)
Tension adjustment of coarse adjustment knobs
While the coarse adjustment motion is normally stiff and heavy, it
is freely adjustable for either heavy or light movement depending
on the observer’s preference. To adjust the tension, rotate the ten-
sion adjustment ring 8 In the direction of the arrow to tighten the
coarse adjustment knobs. (Fig. 27)
* The fine adjustment motion is not adjustable.
* Be careful not to forcibly rotate the coarse or fine adjustment
knobs against the upper or lower limit of the focusing range.
* Do not rotate the coarse and fine adjustment knobs simultane-
ously to avoid any damage to focusing adjustments.
0 Condenser Centration
The centering operations of the long working distance condenser and
the ultra long working distance condenser are the same as follows.
C
1) Set the turret @ to the ” 0 @I ” position. (Fig. 28)
2) Bring the specimen into focus by means of the 10X objective.
* It is recommended to stop down the aperture iris diaphragm 0 :
for easier focusing on an unstained specimen. (Fig. 28)
i
3) Stop down the field iris diaphragm by means of the field iris dia-
phragm lever @ and adjust the condenser height until the image
of the field diaphragm can be observed sharply. (Figs. 28, 29)
* Holding the right condenser knob with the right hand, rotate
the left condenser knob until the tension of the condenser height
adjustment knobs is adjusted to your preference.
4) Bring the image of the field diaphragm into the center of the field
by means of the condenser centering knobs 0. (Ftgs. 28, 29)
Re-open the diaphragm until the small pinhole image of the dia-
phragm becomes a larger polygonal area around the perrphery of the
field. For practical use, slightly open the diaphragm to circumscribe
the field of view.
0 Bulb Centration
After the complete optical setup, center the halogen bulb.
1) Rotate the magnification changer dial @to position “‘CT”. (Fig. 30)
2) Rotate the focus ring @ to focus on the exit pupil of the 10X
objective (located at the same plane as the phase annulus of the
phase objective, the light annulus of the phase contrast condenser
or the aperture iris diaphragm). (Fig. 30)
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