Fig. 33
3) Focus on the phase annulus @ by means of the focus ring 0. (Figs.
32,33)
4) Rotate the condenser turret until the magnification of the objective
engaged appears in the front.
5) Press the light annulus centering knobs @ and rotate them until
both annuli are concentric and superimposed, then slowly disengage
the centering knobs @ (Fig. 34)
C
7
r
6) Rotate the magnification changer @ to the “IX” position, and
observe the specimen to check the phase contrast effect. (Figs. 32,
35)
Fig. 34
Fig. 35
Phase contrast
@ Use of the Correction Collar of the Objectives LWD-CD Plan 20X and
40x.
After coarse and fine adjustments, rotate the correction collar, keeping
the specimen in fine focus until optimum resolution is obtained. Proper
use of this collar is specially effective to prevent the deterioration of
the objective resolution caused by the uneven thickness of various
petri dishes, culture bottles, etc. (Fig. 36)
* The correction collar is effective with a vessel bottom from 0 up
to 2mm in thickness.
1) If the thickness of the vessel bottom is known:
Match the correc;tion collar to the thickness of the vessel bottom by
the collar scale provided,
Fig. 36
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