r
.
Fig. 37
2) If the thickness of the vessel bottom is unknown:
The optimum position for the correction collar can be obtained
from the image resolution. After focusing adjustment, if a satis-
factory sharp image is not obtained, rotate the correction collar
to the right and left so that you can compare the images at both
sides. Reset the collar to the better image; then starting from this
Position, further rotate the collar to the right and left until both
images can be obtained for comparison with each other.
AS YOU
repeat this procedure several times, you have to fine focus each
time the correction collar is rotated.
0 Use of Iris Diaphragms
1) Field iris diaphragm
The field iris diaphragm controls the diameter of the ray bundle
impinging on the specimen surface and thus increases image defini-
tion and reduces glare.
2) Aperture iris diaphragm
In order to achieve optimum objective performance in brightfield,
the opening of the aperture iris diaphragm should be matched to
the N.A. of the objective in use. It is often preferable, however, to
stop down the aperture diaphragm by about 70% to 80% of the
objective N.A. (Fig. 37)
(As seen through eyepiece tube,
with eyepiece removed.)
CD Filters
Optimum use of proper filters enhances the effective observation and
photomicrography.
Drffusron
/
Interference
(green)
Neutral
density (grey)
Light balancing
*Heat absorbing
45WF Eliminates uneven illumination.
43-l F550-W45
43N D25-W45
43N D6-W45
45-LBD-2N
45-HA
Enhances phase contrast.
Reduces light intensity without
changing color temperature.
For color photomrcrography
with daylight film.
Absorbs heat waves 760nm
and higher to protect the speci-
men.
*This filter is built in the IMT2-LSH. It is recommended to add a heat
filter for prolonged observation or time-lapse photography of tissue
cultures, etc.
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