© PSI (Photon Systems Instruments), spol. s r. o.
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Fig. 7 OJIP measurement performed with different intensities of Super pulse.
Actinic pulse setting
Intensity of Actinic pulse should correspond with cultivation light intensity or should be set according to application.
If you see Overflow on display during measurement please lower the intensity of used pulses.
In case of Low value on display during measurement please heighten the intensity of used pulses.
7.2 ME ASUR EM E N T
No device calibration is needed before chlorophyll fluorescence measurement. Results of fluorescence measurement
depend on device settings.
Fluorescence measurement with FluorPen is perform as follows:
• If you want to prepare dark adapted sample (prior for F0, QY, NPQ, LC) place the sample for defined period
on dark place or apply on the plant the detachable leaf-clip which is modified for dark-adaptation. Duration
of sufficient dark-adaptation depends on species, mostly varies between 10 and 15 minutes.
• Turn ON device – hold SET button for 1 sec.
• Place the dark-adapted leaf into the leaf-clip.
• Go to Measure > choose required parameter for example - QY.
• Press SET to confirm.
• FluorPen measures required parameter. If you choose protocol such as OJIP, LC or NPQ the display shows
the percentage of measurements.
• Value of required parameter appears on display after measuring. Result of OJIP, NPQ or LC protocol is a
graph, so all data you will see after data download using FluorPen Software.
• During the measurement all data are stored into the device memory.
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