PSI FluorPen FP 110 User Manual

© PSI (Photon Systems Instruments), spol. s r. o.
24
7.3.3 LIGHT CURVE (LC) PROTOCOL
The protocol called Light Curve (LC) was designed to acquire parameters for construction of Light Response Curve
relating the rate of photosynthesis to photon flux density. The method is based on successive measurements of the
sample exposed to a stepwise increase of light intensity. The effective quantum yields of photosynthesis are determined
under various light intensities of continuous illumination. Measurement is based on pulse modulated fluorometry (PAM).
Several LC protocols are predefined in FP. These differ in number and duration of individual light phases and light
intensities.
# of phases
Phase duration
Light intensities [µmol.m
-2
.s
-1
]
LC1
6
60s
10; 20; 50; 100; 300; 500
LC2
5
30s
100; 200; 300; 500; 1000
LC3
7
60s
10; 20; 50; 100; 300; 500; 1000
Tab. 3 LC Protocols.
The protocol includes following measured and calculated parameters:
Abbreviation
Explanation
F
0
minimum fluorescence in dark-adapted state
F
m
maximum fluorescence in dark-adapted state
F
m
_Ln
‡
maximum fluorescence in light adaptation state
F
t
_Ln
‡
instantaneous fluorescence during light adaptation
QYmax*
maximum quantum yield of PSII in dark-adapted state - Fv/Fm
QY_Ln
‡
**
instantaneous PSII quantum yield induced in light
‡
n represents a sequential number of light phase
*Calculated as (F
m
– F
0
) / F
m
** Calculated as (F
m
_Lx – F
t
_Lx) / F
m
_Lx

PSI FluorPen FP 110 Specifications

General

Brand	PSI
Model	FluorPen FP 110
Category	Measuring Instruments
Language	English