Siemens ADVIA 2400 - Washing the Lines

164 ADVIA 2400 Operator’s Guide
9. Select
Execute
10. When prompted, select
Yes to execute buffer prime.
Washing the lines
1. Remove the buffer bottle with the deionized water and replace it with a bottle filled
with a solution of 475 mL of deionized water and 25 mL probe wash 3 solution.
2. At the ISE Operation window, in the Bufferprime area, enter
50 in the Times field.
3. Select
Execute.
4. When prompted, select
Yes to execute the buffer prime.
Rinsing the lines
1. Replace the probe wash 3 solution bottle with a bottle of deionized water.
2. In the Bufferprime area, enter
50 in the Times field, then select Execute.
3. Remove the dummy electrode.
4. Reinstall the Na, K, and Cl electrodes.
5. In the Initialize area, select
Execute.
6. Before reinstalling the buffer-solution bottle, thoroughly rinse the buffer bottle cap,
float switch, and tube with deionized water and dry completely.
7. Install the buffer bottle or replace it if the volume is low.
Priming and initializing the ISE module
1. At the ISE Operation window, in the Bufferprime area, enter 15 in the Times field.
2. To prime the line with buffer, select
Execute, then select Yes.
3. When the priming is finished, verify that the electrodes are not leaking.
4. At the ISE Operation window, select
Exit, then select Yes.
5. Run 10 pooled serum samples, or do an ISE CV check.
6. Perform calibration and run controls.