31
Figure 3-14. Box filament positioning.
3.6.3.2 Geometry
The box size you select should be approximately 0.5 to 1.5 mm larger than the outside
diameter of the glass you are using. The width of the filament will depend on the research
application. A good general-purpose box filament is the 2.5mm wide, 2.5mm high, and 2.5mm
deep filament (FB255B). Special box filaments made to accommodate larger diameter glass or
special pulling applications are available upon request.
Table 3-4. Box filament sizes.
Filament Description Glass O.D.
FB215B 2mm square x 1.5mm wide 1.0mm
FB220B 2mm square x 2.0mm wide 1.0mm
FB230B 2mm square x 3.0mm wide 1.0mm
FB255B (Standard) 2.5mm square x 2.5mm wide
≤ 2.0mm
FB245B 2.5mm square x 4.5mm wide
≤ 2.0mm
FB315B 3mm square x 1.5mm wide
≤ 1.5mm
FB320B 3mm square x 2.0mm wide
≤ 1.5mm
FB330B 3mm square x 3.0mm wide
≤ 2.0mm or 2-3 barrels
3.6.4 Trough Filament
The trough filament is a general-purpose filament. It is recommended for standard or thin
wall glasses used for patch pipette fabrication, sharp electrodes with long tapers, and some
types of microinjection needles.
Figure 3-15. End view of trough filament and glass.
Note: The Ramp Test value with a trough filament will be lower than that with the box
filament, thus program HEAT values will be correspondingly lower in order to reach similar
operating temperatures.
P-97 FLAMING/BROWN MICROPIPETTE PULLER OPERATION MANUAL – REV. 2.43 - DOM (20161118)
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