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1. GENERAL INFORMATION
The P-97 can fabricate pipettes for use in intracellular recording, patch clamping,
transferring (ICSI, ES Cells), microinjection, aspiration, and microperfusion. Realizing the
full potential of this instrument is dependent on a complete understanding of the way it
implements the pulling process. To this end, we urge that this manual be read in its entirety.
To aid in understanding the function of the instrument, sample programs are already loaded
in memory (as discussed in subsequent material).
The Model P-97 Flaming/Brown Micropipette Puller combines a proven pulling technology
with programmability to produce a very versatile instrument. The pulling mechanism is
derived from the P-77/P-80 series of pullers, which have demonstrated the ability to pull a
complete range of pipette profiles. Added to this mechanism is the ability to program
different pulling sequences; thus, allowing ease of use for pulling a multiplicity of pipettes on
one device.
The P-97 is a ‘velocity sensing’ puller. This patented feature allows the puller to indirectly
sense the viscosity of the glass, giving the P-97 the ability to pull pipettes from all glasses
except quartz. Even difficult to pull formulations, such as aluminosilicate glasses, are
handled with relative ease.
Throughout this manual reference will be made to the size of the glass tubing used to pull
micropipettes. The convention used here for describing the outside diameter (O.D.) and
inside diameter (I.D.) is as follows: O.D. x I.D. These dimensions will always be given in
millimeters (mm). See our catalog or visit
www.sutter.com to find a wide selection of glass
capillaries available for purchase.
1.1 What’s New on the P-97?
For users familiar with the P-80PC and/or P-87 pullers, the P-97 provides the advanced
features incorporated into those instruments plus:
• Humidity controlled chamber surrounding the heating filament. Glass is now pulled in a
constant humidity environment to minimize environmental effects on pipette
reproducibility.
• Vacuum fluorescent display for improved readability
• 25% increase in available power
• Programmable air pressure
• Time of pull displayed at the end of each pull sequence
• Write protection to prevent programs from being corrupted
• Displayed date/time of last program edit
• Increased memory capable of storing 100 programs
• Switchable cooling mode (Time and Delay) to facilitate easy transfer of programs from
both P-80PC and P-87 pullers
P-97 FLAMING/BROWN MICROPIPETTE PULLER OPERATION MANUAL – REV. 2.43 - DOM (20161118)
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