Operation
SORVALL
®
Centrifuges
4-12
So that runs are not inadvertently corrupted, whenever STEP
RUN has been selected (indicator lit), changes to run parameters
will be ignored unless STEP RUN is deselected before START
is pressed. Because of this, the only changes that could be made
without deselecting would be if a different step run was recalled
from program memory (program numbers 10-15).
After STEP RUN selection, SAVE TO PROGRAM MEMORY?
will appear in the display. For future use, step runs may be saved
to program memory, and can occupy program number 10-15.
SAVE TO PROGRAM MEMORY?
This screen allows pre-programming of up to 15 different sets of
run parameters (9 standard runs, and 6 step runs) for simple recall
and error-free run reproducibility of frequently used protocols.
To eliminate inadvertent loss of existing programs, the storage
sequence alerts users before overwriting. The convenient RE-
CALL key allows browsing through existing programs for
selection or reference.
NOTE If you plan to use the QC RUN feature, specific guidelines
should be considered when saving parameters to memory
(see QC RUN on page 3-8).
To save a run, all run parameter selections should be made before
selecting this option. After SAVE TO PROGRAM MEMORY
selection and assignment of a program number, the SET display
will return to the HOME screen. See Programmed Operation on
page 4-4.
Temperature Control
The RC12BP™ controls calculated sample temperature during a run
based on the rotor selected, rotor speed, run time, SET temperature
and measured chamber temperature. The complex calculation the
centrifuge performs must assume, however, that the sample and the
rotor are at SET temperature at the start of the run. The centrifuge
makes precise calculated adjustments to control temperature of
equilibrated components; the control adjustments will not precisely
compensate for the error introduced if a rotor or its contents are not
at SET temperature at the start of a run (although, eventually,
components will equilibrate and control will be appropriate).
NOTE When temperature control is critical, the sample, SET
temperature, rotor (body, buckets, adapters, and cover),
and rotor chamber should all be at the same tempera-
ture when START is pressed.
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