RC12
BP™
Operation
4-13
To equilibrate rotor temperature before operation, either store the
rotor in a controlled temperature environment (such as refrigerator or
cold room), or precool/preheat the rotor in the centrifuge chamber,
until the rotor is the same temperature as the sample and the required
SET temperature.
Temperature control needs can vary with the application. In many
cases, the RC12BP™ Centrifuge will be used to run large volumes of
sample for short amounts of time. Over the course of a short run, if all
components have been equilibrated, it would be difficult to signifi-
cantly change the sample temperature – even if the centrifuge's
temperature control performance was reduced.
Rotor Temperature Equilibration
To equilibrate the rotor temperature in the centrifuge chamber:
1. Install the rotor in the rotor chamber, place empty buckets in all
positions, and install the rotor cover.
2. Close the chamber door.
3. Turn off all options (access with MENU key).
4. (Future use, if rotors are added) Check to be sure that the correct
rotor name is in the ROTOR field of the SET display – if not,
press ROTOR until it is.
5. Press SPEED. If you are precooling the rotor, input 1500 rpm; if
you are preheating the rotor, input the rotor's maximum rated
speed (the maximum rated speed of an H-12000 rotor is 4700
rpm).
6. Press HOLD to select a continuous run.
7. Press TEMP and enter the desired rotor temperature.
8. Press START to begin rotor temperature equilibration. The
centrifuge will alert you that a sample overtemperature or under-
temperature condition exists, and that pre-cooling or pre-heating
is taking place – press CLEAR.
9. Monitor progress of the value in the RUN TEMPERATURE
display, and note the time when the displayed RUN temperature
reaches the SET temperature. From when it does, allow the run
to continue for an additional 30 minutes, then press STOP.
To Next Page
Loading...
