17
1st order linear: Concentration = Intercept+ Slope*Absorbance;
1
st
order linear through zero: Concentration = Slope*Absorbance;
Press Enter to confirm fitness method selection. The screen returns to previous menu and
the * move to “Measure Standard” automatically.
Important Note:
At least two standard solutions are required to establish 1
st
order linear through
zero curve. If only one standard solution available you must select 1
st
order linear.
8. Measure standard solutions:
8.1 Press ENTER at “Measure Standard” The following screen will pop up.
8.2 Place the cuvette with reference solution in the cuvette holder, press 【0Abs/100%T】to
blank. After blank is complete it is ready for standard solution measurement starting
from STD. No.1
8.3 Place standard No. 1 in the cuvette holder and make sure it is in the optical path
(working position). Enter the known concentration value. Refer to Intercept value
entry for data entry procedures. Check and make sure the value entered matches
the value of the standard No.1 (it is usually labeled on the standard).
8.4 Press ENTER to measure the standard No.1. The Absorbance of the standard
solution No.1 will be measured and displayed. Since this is the first measurement
the absorbance is marked “A1”
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