Zeiss AxioObserver D1 - Post-Acquisition Adjustments; Brightness and Contrast Tuning; Adding a Scale Bar; Saving Image Files

4. Repeatexposuretimemeasurementforeachchannel,however,adjustthefinefocusforonlyone
channeliethemostimportantone
5. Tocaptureyourfinalimage,pressthebuttonlocatedatthebottomoftheCtab.
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Post‐AcquisitionAdjustments
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Brightness/Contrast
1. Temporarilyturnoffthepseudo‐coloringofthedisplayedimagebypressingthebutton.Each
channelisdisplayedinblack/white;youcanviewonechannelatatime.
2. Clickonthepropertiesbuttonfoundbelowtheimage
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3. WhileunderDisplaytabofpropertieswindow,clicktheMin/Maxbutton,orunderyourimage,press
thebutton.
4. Youcanmakefurtheradjustmentsusingthedisplayedhistogram(iemovetopoflineleft,andbottom
oflineright)
‐ NOTE:donotadjusttheGamma
5. Repeatforeachchannel
6. Pressthebutton
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AddingaScalebar
1. Toaddascalebar,pressthebutton,orAnnotationsfromthemainmenuandselectScalebar.
2. Dragcursoralongimagetogetdesiredscalebarsize
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SavingFiles
1. Fromthemainmenu,selectFileSaveas
2. SaveyourfilesintheAxioVisionformat(.zvi)intheappropriatedestinationyourlabfolderyour
folder.PleaserefertotheAttentionUserssignspostedonthewall.
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ShutDownProcedure
8 Ifusedoil,gentlyremoveoilfromobjectiveusingneatlyfoldeddrylenspaper
9 Put10xobjectiveinplace,andlowerobjective
10 CloseAxioVisionprogram.Donotsavechangesmadetotheexperiment!
11 Shutdowncomputer.Waituntilcompletelyturnedoff
12 TurnMicroscopeOFF
13 Toggle
PowerBarswitchOFF
14 FluoArcLamp:
‐ Regularworkhours:setintensityto50%oncontrolpad(F2),andleavelampON
‐ Non‐workhours:TurnlampOFF
15 Covermicroscopewithdustcover,butDON’TCOVERTHEHOTLAMPHOUSING!!
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