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General| Microscope Type | Inverted Microscope |
|---|---|
| Software | ZEN software |
| Optical System | Infinite optical system |
| Illumination | LED |
| Contrast Methods | Phase Contrast, DIC, Fluorescence |
| Stage | Motorized stage or manual stage |
| Camera Compatibility | Various scientific cameras |
| Camera Ports | C-mount |
| Automation | Motorized components available for automated imaging |
| Objectives | Plan Apochromat and Plan Achromat |
| Filter Sets | Multiple filter sets for fluorescence |
| Focus Drive | Motorized focus drive |
| Dimensions | Varies depending on configuration |
| Weight | Varies depending on configuration |
Steps to power on the microscope and associated components.
Logging into the computer and opening the AxioVision software.
Cleaning the objective and setting the 10x objective.
Steps to shut down the computer, microscope, and lamp.
Properly covering the microscope after shutdown, avoiding the lamp housing.
Accessing and selecting multidimensional acquisition settings.
Loading experiment setups and configuring imaging channels and colors.
Preparing the slide and placing it on the microscope stage.
Bringing the specimen into focus and centering the area of interest.
Using the Measure button for live preview and selecting display range.
Adjusting exposure time for optimal signal intensity.
Adjusting image brightness and contrast using histogram and properties.
Inserting a scale bar for image measurement.
Saving captured images in the AxioVision format (.zvi).
Details of the microscope's display screen elements.
Controls for the transmitted light path and lamp.
Controls for the reflected light path and components.
Description of different light path configurations.
Details on 10x, 20x A-Plan, 20x LD Plan-NeoFluar, and 20x Plan-Apochromat objectives.
Details on 40x, 63x, and 5x objectives including immersion and DIC.
Specifications for the 100x Plan-NeoFluar oil objective.
Information regarding the transmitted light filter.
Excitation, beam splitter, and emission wavelengths for DAPI/BFP.
Excitation, beam splitter, and emission wavelengths for Cy2/GFP.
Excitation, beam splitter, and emission wavelengths for YFP.
Excitation, beam splitter, and emission wavelengths for Cy3.5/mcherry.
Excitation, beam splitter, and emission wavelengths for Cy5.
Guidelines for using and turning the FluoArc lamp on/off.
Instructions for cleaning and using oil objectives.
Rules for booking time and logging station usage.
Guidelines for saving and backing up experimental data.
Permitted uses of the computer for image acquisition only.
Detailed rules for proper immersion oil application and cleanup.
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