3M Petrifilm 6475 - Instructions for Use; Sample Preparation; Plating; Incubation

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(English)
EN
LIMITATION OF WARRANTIES / LIMITED REMEDY
EXCEPTASEXPRESSLYSTATEDINALIMITEDWARRANTYSECTIONOFINDIVIDUALPRODUCTPACKAGING,3MDISCLAIMSALLEXPRESSANDIMPLIED
WARRANTIES,INCLUDINGBUTNOTLIMITEDTO,ANYWARRANTIESOFMERCHANTABILITYORFITNESSFORAPARTICULARUSE.Ifany3MFoodSafety
Productisdefective,3Moritsauthorizeddistributorwill,atitsoption,replaceorrefundthepurchasepriceoftheproduct.Theseareyourexclusive
remedies.Youmustpromptlynotify3Mwithinsixtydaysofdiscoveryofanysuspecteddefectsinaproductandreturnitto3M.PleasecallCustomer
Service(1-800-328-1671intheU.S.)oryourofcial3MFoodSafetyrepresentativeforaReturnedGoodsAuthorization.
LIMITATION OF 3MLIABILITY
3MWILLNOTBELIABLEFORANYLOSSORDAMAGES,WHETHERDIRECT,INDIRECT,SPECIAL,INCIDENTALORCONSEQUENTIALDAMAGES,
INCLUDINGBUTNOTLIMITEDTOLOSTPROFITS.Innoeventshall3M’sliabilityunderanylegaltheoryexceedthepurchasepriceoftheproduct
alleged to be defective.
STORAGE
Store unopened 3MPetrilm RYM Plate pouches refrigerated or frozen (-20 to 8°C / -4 to 46°F). Just prior to use, allow unopened pouches to come
toroomtemperaturebeforeopening(20-25°C/<60%RH).Returnunused3MPetrilmRYMPlatestopouch.Sealbyfoldingtheendofthepouch
over and applying adhesive tape. To prevent exposure to moisture, do not refrigerate opened pouches. Store resealed pouches in a cool dry place
(20-25°C/<60%RH)fornolongerthan4weeks.Itisrecommendedthatresealedpouchesof3MPetrilmRYMPlatesbestoredinafreezer(see
below)ifthelaboratorytemperatureexceeds25ºC(77ºF)and/orthelaboratoryislocatedinaregionwheretherelativehumidityexceeds60%(with
theexceptionofair-conditionedpremises).
To store opened pouches in a freezer, place 3MPetrilm RYM Plates in a sealable container. To remove frozen 3MPetrilm RYM Plates for use, open
the container, remove the plates that are needed and immediately return remaining plates to the freezer in the sealed container. Allow 3MPetrilm
RYMPlatestocometoroomtemperaturebeforeplating.3MPetrilmRYMPlatesshouldnotbeusedpasttheirexpirationdate.Donotstoreopen
pouchesinafreezerwithanautomaticdefrostcycle,asthiscoulddamagethe3MPetrilmRYMPlatesduetorepeatedexposuretomoisture.
Donotuse3MPetrilmRYMPlatesthatshowdiscoloration.Expirationdateandlotnumberarenotedoneachpackageof3MPetrilmRYMPlates.
The lot number is also noted on individual 3MPetrilm RYM Plates.
DISPOSAL
After use, 3MPetrilm RYM Plates may contain microorganisms that may be a potential biohazard. Follow current industry standards for disposal.
Forinformationonpotentialbiohazards,referenceBiosafetyinMicrobiologicalandBiomedicalLaboratories,5
th
edition,SectionVIII-B:FungalAgents
orequivalent.
INSTRUCTIONS FOR USE
Follow all Product Instructions carefully. Failure to do so may lead to inaccurate results.
Wearappropriateprotectiveapparelandfollowstandardgoodlaboratorysafetypractices(GLP).
1
Sample Preparation
1. Prepare appropriate dilution(s) of the sample as needed.
 Useappropriatesterilediluents:
 Buttereld’sphosphatebuffer(ISO5541-1),BufferedPeptoneWater(ISO),0.1%peptonewater,peptonesaltdiluent,salinesolution(0.85-0.90%),
bisulte-free letheen broth, or distilled water. Do not use diluents containing citrate, bisulte or thiosulfate with 3MPetrilm RYM Plates;
they can inhibit growth. If citrate buffer is indicated in the standard procedure, substitute with 0.1% peptone water, warmed to 40-45°C.
 See“SpecicInstructionsforValidatedMethods“forspecicrequirements.
2. Blendorhomogenizesample.
Plating
1. Placethe3MPetrilmRYMPlateonaat,levelsurface.
2. Liftthetoplmandwiththepipetteperpendiculardispense1mLofsamplesuspensionontothecenterofbottomlm.
3. Roll the top lm down onto the sample.
4. Placethe3M™Petrilm™FlatSpreader(6425)orotheratspreaderonthecenterofthe3MPetrilmRYMPlate.Pressgentlyonthecenterof
the spreader to distribute the sample evenly. Spread the inoculum over the entire 3MPetrilm RYM Plate growth area before the gel is formed. Do
not slide the spreader across the lm.
5. Remove the 3MPetrilm Flat Spreader and leave the 3MPetrilm RYM Plate undisturbed for at least one minute to permit the gel to form.
Incubation
Incubate3MPetrilmRYMPlatesat25°C+/-1°Cor28°C+/-1°Cfor48+/-2hours*inahorizontalpositionwiththeclearsideupinstacksofno
more than 40.
*If colonies appear faint, allow an additional 12 hours of incubation time for enhanced interpretation. If a 60 hour time-point for interpretation is not
convenient,extendingtheincubationtimeto72hoursisanacceptablealternative.
See“SpecicInstructionsforValidatedMethods“forspecicrequirements.