4Deep HoloSea S7 - 6 Appendix; 6.1 Principle of Operation

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5 TROUBLESHOOTING HoloSea S7

5 Troubleshooting

Issue Solution

Camera not connecting Check that the cables are

properly attached, and power

is on

If there are more than one

Ethernet ports on the

computer, try a different one

If you are using a laptop

computer docking station,

use the Ethernet port on the

docking station (not the

laptop itself)

No light from point source/ not much light from point source If there is no light coming

from the point source and it

is properly connected there

may be a problem with the

laser, there may be direct

damage to the laser, or the

laser diode may have burned

out. Send the microscope or

point source back to the

manufacturer for diagnosis.

Background light must be

reduced to obtain optimal

images.

Table 1: Common troubleshooting problems and solutions

6 Appendix

6.1 Principle of Operation

The HoloSea operates on the principles of holography to image a volume in magniﬁcation. A 405

nm laser from a ﬁbre optic cable produces a spatially coherent light source as a reference wave.

Light scattering from the objects within the media (water) will interfere with the reference wave

to produce an interference pattern which contains spatial and phase information of the objects

within the volume. This interference pattern, the hologram, is recorded by a CCD camera, and

reconstructed mathematically to build images of the objects within the volume.