HoloSea S7 1 OVERVIEW
1 Overview
The HoloSea has been developed to operate in conjunction with 4Deep’s software (Octopus,
Swordfish or Stingray). Note that hologram collection cannot be completed without the use of
4Deep software.
This user guide is strictly related to the installation, use, care of and functionality of the HoloSea.
For a detailed description of the setup and operation of the 4Deep software, please refer to the
respective user guides.
The HoloSea is a submersible holographic microscope; for the benchtop microscope, see the
Desktop Microscope.
The HoloSea can be used to collect images from the fields of:
• Marine research: water profiling, algae, plankton, phytoplankton
• Oil & gas
• Water quality and monitoring: microorganism imaging
• Ocean research
• Algae production: algae profiling
• Counting, data and morphological analysis, quantitative phase analysis: many other applica-
tions
1.1 Purpose
The HoloSea comes from a suite of instruments that all operate under proprietary digital inline
holography technology platform, developed by 4Deep. With its new and unique method of data
acquisition and analysis, this system is designed to optimize your research of the microscopic
world. The microscope is able to capture dynamic movement and is useful for time-dependent
experiments. The HoloSea allows you to quickly and easily observe micro-organisms and particles
up to a depth of 5 kilometres (in custom models).
The measurement technique used is digital inline holographic microscopy in transmission. The
light source is a single source of 405 nm.
1.2 Benefits
Compared to a traditional microscope, the holographic microscope has many benefits:
• Real-time, in-situ capability to observe of particles and micro-organisms in all aquatic envi-
ronments
• Portable and lightweight, making it easy to deploy, yet rugged
• High-flow capabilities; an be towed at 8 knots (4 m/s), or stationed in rivers
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