Abaxis VetScan HM5 - Intrinsic Sample Interferences

C-2 Potential Sample Interferences
Table C-1: Intrinsic Substances: Potential Sample-Induced Interferences
Sample Condition Indicators Results Affected Causes Solutions Lipemia • Cloudy, white plasma.
• High MCHC.
Increased HGB, MCHC, MCH. RBCs may be smudged on blood film.
Non-fasted sample Redraw fasted sample. Metabolic disorder
a
Use RBC, PCV, MCV, and RDW rather than HGB, MCH, MCHC values to assess anemia.
Hemolysis Pink or red plasma. • Decreased RBC,
PCV
• Increased MCHC
Traumatic venipuncture
Redraw w/ clean venipuncture. Remove needle before dispensing blood into tube.
Hemolytic anemia N/A
Clumped platelets • Decreased platelet count w/ platelet clumps
often visible on blood smear or applicator stick dipped in sample.
• Rising left side of lymphocyte curve. • Small pale clots stick to wooden applicator
stick swirled in sample.
• Low platelet histogram with a rising right
side.
• Possible tailing on right side of granulocyte
curve.
Decreased PLT +/- increased WBC
Traumatic venipuncture or feline species
Redraw; collect blood in anti-coagulated syringe or use vacutainer system; invert tube several times immediately after filling; vortex sample immediately before testing.
Excess potassium EDTA (under-filled tube)
Fill tube at least halfway, or remove portion of potassium EDTA before filling tube.
Miscellaneous/ idiopathic
Use alternative anti-coagulant as heparin or citrate; vortex sample immediately prior to sampling.
Giant platelets Right side of platelet histogram runs into RBC
histogram.
Decreased PLT, decreased MPV
Thrombopoiesis, feline species
Confirm platelet estimate on smear and/or manual platelet count.
Clotted sample • Visible clot in sample.
• Red clot(s) stick to wooden applicator stick
swirled in sample.
• Platelet clumps may or may not be visible
on blood smear.
Decreased PLT, decreased WBC, and/or decreased RBC (varies with clot size)
Traumatic venipuncture and/or delayed transfer to anti-coagulant
Redraw with clean venipuncture; use vacutainer system or anti-coagulated syringe; mix collection tube by multiple inversions immediately after filling. Clotted sample may clog the sample needle.
Lyse-resistant RBCs • Rising left side of lymphocyte curve.
• L flag in dogs.
Increased WBC, increased Lymphs
Idiopathic (most commonly with felines)
Lyse volume may be adjusted for the sample (for additional information, contact Abaxis Technical Support — see page 1-3).
a).Diabetes mellitus, nephrotic syndrome, hypothyroidism, lipoprotein lipase deficiency, acute pancreatitis, cholestasis, hyperadrenocorticism, hypercholesterolemia in briards, idiopathic hyperlipidemia of miniature schnauzers.