44 Advanced Operation
3 Method Development
• The HS will start the method, running one vial at a
time, until it would exceed the specified upper limit on
the parameter.
• View the current method parameters by pressing
[Temp], [Time], or [Vial]. As the HS increments the
method parameter for each new vial, the new value is
displayed.
Oven temperature
When incrementing oven temperature, consider the following:
• Higher temperatures generally improve peak areas.
• Do not exceed the solvent (or analyte) boiling point.
• Incrementing temperature can increase throughput.
• All thermal zones increment at the same rate. If a heated
zone reaches (or would exceed) its maximum temperature,
it will hold at its maximum temperature for any
remaining vials. For example, consider an oven
temperature of 250 °C with a transfer line temperature of
275 °C. If incrementing 10 °C, on the third run, transfer
line temperature would be 305 °C while the oven would
be at 280 °C. The maximum temperature of the transfer
line would be exceeded. Instead, the temperature holds at
300 °C. See the example in Table 10 below.
• Vials in this case are run in series. There is no overlap
since the oven temperature differs for each vial.
• Do not enter a series that exceeds the number of
available vials in the tray (or carousel).
Table 10 Example temperatures, in °C, during parameter increment of
10°C per step
Oven Transfer line Sample loop
250 275 265
260 285 275
270 295 285
280 300 295
290 300 300
300 300 300
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