viii • Table of Contents
MultiClamp 700A Theory and Operation, Copyright 2000, 2001 Axon Instruments, Inc.
CHAPTER 4: A PRACTICAL GUIDE TO ELECTROPHYSIOLOGICAL
RECORDING USING THE MULTICLAMP 700A ....................31
General Advice........................................................................................................32
Chamber Design ..................................................................................................32
Interfacing a Computer........................................................................................34
Patch Clamping .......................................................................................................35
Headstage and Holder Considerations.................................................................35
Forming a Gigaseal..............................................................................................36
Whole-cell Voltage Clamp Recording ................................................................39
Perforated-patch Recording.................................................................................42
Low Noise Techniques ........................................................................................43
Sharp Microelectrode Recording.............................................................................47
Sharp Microelectrode or Patch Electrode? ..........................................................48
Microelectrode Properties ...................................................................................48
Filling Solutions ..................................................................................................52
Impaling Cells .....................................................................................................52
CHAPTER 5: REFERENCE SECTION................................................................55
Audio Monitor.........................................................................................................56
Bath Headstage and Electrodes ...............................................................................60
Bridge Balance ........................................................................................................63
Buzz.........................................................................................................................66
Capacitance Compensation......................................................................................67
Capacitance Neutralization......................................................................................73
Clear ........................................................................................................................76
Electrochemistry......................................................................................................77
External Command Inputs.......................................................................................79
Feedback Resistor....................................................................................................81
Filters.......................................................................................................................83
Grounding and Hum................................................................................................87
Headstage ................................................................................................................89
Help .........................................................................................................................94
Holders ....................................................................................................................95
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