14 • Tutorials
MultiClamp 700A Theory and Operation, Copyright 2000, 2001 Axon Instruments, Inc.
3. Piece of aluminum foil or a metal container, such as a coffee can (in which to place
the model cell) grounded to the 4 mm Signal Ground plug on the rear of the
MultiClamp 700A.
4. Oscilloscope, connected to the MultiClamp 700A by means of a BNC cable, with
which to monitor the output. The tutorials will assume an oscilloscope is being
used. Alternatively, the Scope window of Clampex could be used to monitor the
output.
Model Cell
All of these tutorials use the PATCH-1U model cell, which contains simple circuits of
resistors and capacitors designed to simulate three patch clamp recording conditions:
(1) Pipette in the bath (Connector labeled BATH on the model cell), (2) Gigaseal
(PATCH), and (3) Whole-cell (CELL). The circuit for each of these is as follows.
(Also see
MODEL CELL in Chapter 5.)
BATH: 10 M "electrode" resistor to ground.
PATCH: 10 G "patch" resistor to ground.
Approximately 5 pF stray capacitance to ground.
CELL: 10 M "electrode" resistor.
500 M "cell membrane" resistor in parallel with 33 pF "cell membrane"
capacitor.
Approximately 5 pF stray capacitance to ground.
Tutorial 1 – Electrode in the Bath: Voltage Clamp
1. Switch on the MultiClamp 700A and run the MultiClamp Commander by double-
clicking on the shortcut icon on the desktop of the PC. Press the toolbar button
labeled with the arrowed circle (Reset to Program Defaults).
Figure 2.1
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