Axon MultiClamp 700A - Chapter 3 Using the MultiClamp 700 A

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Tutorials • 13

Chapter 3

Using the MultiClamp 700A – Tutorials

The purpose of this chapter is to lead the user through the basics of patch clamping and

‘sharp’ microelectrode recording, using the PATCH-1U model cell that comes with the

MultiClamp 700A. The tutorials are designed to illustrate the operation of the

MultiClamp 700A and the MultiClamp Commander. Although this chapter is

primarily directed at inexperienced electrophysiologists, it may also be useful for

experienced researchers who desire a simple introduction to the features of the

MultiClamp 700A.

It is recommended that the tutorials are done in order, because later tutorials assume

knowledge gained in earlier ones.

Check List

These tutorials require the following:

1. MultiClamp 700A main unit, plus one or (optionally) two CV-7A headstages,

connected to a PC as described in Chapter 2. The tutorials will assume that only

Headstage 1 is being tested.

2. PATCH-1U model cell.

Axon MultiClamp 700A - Chapter 3 Using the MultiClamp 700 A - Tutorials; Check List