98 • Reference Section
MultiClamp 700A Theory and Operation, Copyright 2000, 2001 Axon Instruments, Inc.
Heat smoothing the back end of the electrode extends the life of the chloride
coating by minimizing the amount of scratch damage. Another way to protect
the AgCl coating is to slip a perforated Teflon tube over the chlorided region.
Holder Maintenance
Cleaning
For lowest noise, keep the holder clean. Frequently rinse the holder with
distilled water. If more thorough cleaning is required, briefly wash in ethanol
or mild soapy water. Never use methanol or strong solvents.
Replacing the Silver Wire
To replace the silver wire, insert the nonchlorided end through the hole of the
silicone seal and bend the last 1 mm of wire over to an angle of 90°. Press the
wire into the back of the barrel making sure that the silicone seal is flush with
the back of the barrel. Slip the threaded collar over the back of the barrel.
With the large end of the pin directed toward the bent-over wire, screw the pin
cap down firmly, but without excessive force. This assures good electrical
contact.
Adapters
HLR-U right-angle adapters allow the HL-U series holder to emerge at 90° from the
headstage. Use the HLR-U with the HL-U holder.
HLB-U BNC-to-Axon adapter allows conventional BNC-type holders to be used with
Axon U-type headstages. Use the HLB-U with all U-type CV and HS headstages.
These headstages have a threaded white Teflon collet.
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