2 • Introduction
MultiClamp 700A Theory and Operation, Copyright 2000, 2001 Axon Instruments, Inc.
The MultiClamp Commander allows much greater functionality, without the limits
imposed by the amount of space available on the front panel for knobs and switches.
Also, computer control permits “smart” features, such as automatic capacitance
compensation and automatic bridge balance. Telegraph information, performed
through software messaging, includes Gain, Filter and Capacitance, as well as other
useful information such as Scaling Factors and recording Mode.
The Commander interface is completely independent of other software. Thus, the
MultiClamp 700A can be used with any data acquisition package. It is, of course,
compatible with all Digidata series digitizers and with pCLAMP 7 (and above)
software. (However, telegraphing is only supported in pCLAMP versions 8 and
higher.) For third-party software, see our webpage “Developer Info” for a detailed
Software Development Kit that describes how to read telegraph information.
We recognize that software control of an amplifier is an unusual step forward for some
users. If computer mouse control is unsettling, consider the optional SoftPanel device
to control the MultiClamp 700A. The SoftPanel is essentially a hardware extension of
the MultiClamp Commander software. Knobs and buttons replace the mouse “glider”
and clicks. For more information, visit our website or call Axon Technical Support.
The MultiClamp 700A is a sophisticated instrument. Experienced and inexperienced
researchers alike are advised to read this manual thoroughly and to familiarize
themselves with the instrument using model electrodes (i.e. resistors) and model cells
(i.e. parallel RC circuits) before attempting experiments with real microelectrodes and
cells. PATCH -1U model cells are provided for performing the Functional Checkout
(see page 6), and Tutorials (see page 13).
We will be pleased to answer any questions regarding the theory and use of the
MultiClamp 700A. Any comments and suggestions on the use and design of the
MultiClamp 700A will be much appreciated. We welcome reprints of papers
describing work performed with the MultiClamp 700A. Keeping abreast of research
performed helps us to design our instruments to be of maximum usefulness to those
who use them.
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