Axon MultiClamp 700A User Manual

Installation and Basic Operation • 11
Chapter 2
3. Check Seal Test and set the amplitude to 100 mV, and frequency to 50 Hz.
4. Set the Scaled Output filter to 30 kHz.
5. Press Auto Cp Fast to remove the electrode capacitance transient. The
waveform should be a square wave with about 100% overshoot settling to
the baseline in about 1 to 2 ms. The rise time to the peak of the overshoot
should be about 25 µs.
6. Set the Scaled Output filter to 2 kHz.
7. Measure the step response amplitude following the transient. This should
be 500 mV
p-p
±50 mV.
5 G Range
1. Change the feedback resistor to 5 G.
2. Press Auto Cp Fast.
3. The step response should be ~50 mV
p-p
±5 mV.
500 M Range
1. Press the “Reset to Program Defaults” button. (By default, the 500 M
range is selected.)
2. Check Seal Test and set the amplitude to 25 mV.
3. Plug the CELL connector of the PATCH-1U model cell into the CV-7A
headstage.
4. Press the Auto Whole Cell button and then the Auto Cp Fast button.
5. The step response should be ~25 mV
p-p
.
50 M Range
1. Change the feedback resistor to 50 M.
2. Increase Output Gain to 10.
3. Press Auto Whole Cell and Auto Cp Fast.
4. The step response should be ~25 mV
p-p
.

Axon MultiClamp 700A Specifications

General

Brand	Axon
Model	MultiClamp 700A
Category	Amplifier
Language	English