Axon MultiClamp 700A User Manual

30 • Tutorials
MultiClamp 700A Theory and Operation, Copyright 2000, 2001 Axon Instruments, Inc.
7. Reduce Pipette Capacitance Neutralization until oscillations stop, and switch off
Tuning. Similar to voltage clamp mode, the Pulse button in current clamp allows
you to apply on-off current steps. Experiment with different settings for Pulse
amplitude (nA) and duration (ms) while monitoring the effect on Scaled Output.
Figure 2.28
8. Switch on both Holding and Tuning. Observe Scaled Output on the oscilloscope
while pressing the I=0 button.
Figure 2.29
I=0 is a special mode of current clamp in which all command inputs are
disconnected. With the model cell, the Scaled Output (Membrane Potential)
returns to near 0 mV when I=0 is pressed. In a real cell the Membrane Potential
would return to the resting potential of the cell. See
IMPALING CELLS in
Chapter 4 for detailed information on current clamp experiments with real cells.

Axon MultiClamp 700A Specifications

General

Brand	Axon
Model	MultiClamp 700A
Category	Amplifier
Language	English