BMG LABTECH Omega Operating Manual
2014-05-16 0415B0001G 17/29
5.2.1 Fluorescence Intensity Optics
The fluorescence intensity and time-resolved fluorescence light guides are silver in color, liquid-filled and
come connected to the excitation and emission positioning wheels. The yellow marked light guide routes the
excitation light to the sample while the blue marked light guide routes the emission light to the detector.
In addition, the Fluorescence Intensity Optic can be used to measure luminescence. To do so the emission
light guide is used to route the light from the sample to the detector. The luminescence option is of courses
needed for reading luminescence.
If the AlphaScreen option is present, this optic can be used to detect AlphaScreen.
To position the measurement head with the holders, see chapter 5.2 Installation and Changing of Optics.
If the reagent injection option is present, be careful when positioning the needles in the measurement head
(see figure 10) in order to avoid damage to the reagent needles and to the optic.
Figure 9: Fluorescence intensity optic Figure 10: Holes to position reagent needles in the
measurement head
5.2.2 Luminescence Optics
The Luminescence Optics are important for LUMIstar Omegas, but
can also be used in FLUOstar or POLARstar Omegas. The
luminescence optic has one light guide, which is silver in color. The
light guide connects to the emission side. To position the
measurement head with the holders, see chapter 5.2 Installation and
Changing of Optics.
There are two dedicated luminescence optics: a 3 mm diameter light
guide that is used for plates up to 96-wells, and a 2 mm diameter light
guide that is used for plates up to 384-wells.
For reading luminescence, the luminescence option needs to be
present in the FLUOstar or POLARstar Omega.
Figure 11: Luminescence optic
To Next Page
Loading...
