Omega Operating Manual BMG LABTECH
26/29 0415B0001G 2014-05-16
Next to the axle in the center of the housing is a small positioning pin. This pin must fit into one of the holes
on the back of the filter wheel. Replace the filter wheel on the axle and push it into position (the axle should
stick out 3mm). Turning the filter wheel quickly might help position it correctly. Place the filter wheel nut on
the axle and tighten it with your fingers until it is fixed. Spin the wheel again. The filter wheel should move
freely and without vibration. If the wheel seems to drag or wobble, tighten the nut further, or remove the nut
and reposition the wheel.
Replace the cover and reconnect the light guides.
If the instrument makes a grinding sound it is very likely that the large filter wheel nut in
the middle should be tightened further, or that a filter screw is loose.
5.3.2 Fluorescence Filters
The fluorescence filters have a bandwidth that varies depending upon the type of fluorescence filter. Filters
optimized for specific fluorophores can vary in the bandwidth from around 10 nm to around 30 to 40 nm
(measured at ½ height). BMG-10 filters are symmetrical filters that in ½ height have a bandwidth of 10 nm
and hence can be used in both the excitation and the emission position.
5.3.3 Fluorescence Polarization Filters
The excitation filters can go in any available position in the excitation wheel. The emission wheel has two
filters of the same wavelength for each channel (one for parallel and one for perpendicular). They must be
positioned 180° from each other (for example position 3 and position 7 for the 520 filter). The filter
configuration can be entered in ‘Settings | Filters’.
5.3.4 Dual Emission Filters
As for fluorescence polarization, the excitation filter can be placed in any position on the filter wheel. The
emission filters to be used in dual emission measurement must be positioned 180° from each other
(emission position 1 pairs with position 5, position 2 pairs with position 6, etc.).
5.3.5 Absorbance Filters
Absorbance filters are only needed in readers that use PMT based absorbance. Place the filter (either a
BMG-10 or an ‘Abs’ filter) that matches the wavelength of choice in any excitation position. The light goes
through the ‘excitation filter’ into the absorbance optic. It then passes through the sample and is collected in
the bottom optic. Hereafter the light passes through the ‘emission filter’ wheel to the detector. Depending on
your reader options, the following settings must be used:
Reader with Luminescence option:
A lens is mounted into emission filter position 8. This lens can also be used for the
‘emission path’ of the absorbance measurement. Please type ‘lens’ into emission position 8
of the filter table.
Reader without Luminescence option:
Define a filter position (typically position 8) as empty so that all the light from the reaction
will pass the emission filter wheel directly to the detector (‘empty’ needs to be typed into
the filter table and the corresponding emission filter wheel position must be empty).
Use of an additional filter:
You may choose to use an additional second filter beside the one in the excitation filter
wheel to block any stray light. The filter can be used in any emission filter position and
must be selected properly in the protocol definition.
5.3.6 Luminescence Filters
Like for fluorescence filters, the luminescence filters are designed to transmit as much light as possible. The
bandwidth varies depending upon the type of assay (filters are optimized to return the best result) and can
vary in bandwidth from around 10 nm to around 100 nm (measured at ½ height).
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