BMG Labtech FLUOstar Omega - Technical Specifications; Measurement Principles; Reagent Injection

BMG LABTECH Omega Operating Manual
2014-05-16 0415B0001G 5/29
1 Technical Specifications
Measurement principles
FLUOstar Omega: - Fluorescence intensity
- Time-resolved fluorescence – TR-FRET (opt.)
- Luminescence (opt.)
- UV/VIS absorbance spectra
- AlphaScreen
®
/ AlphaLISA
®
(opt.)
- Upgradeable to POLARstar Omega
LUMIstar Omega: - Luminescence
- Simultaneous dual emission luminescence (opt.)
- Upgradeable to FLUOstar / POLARstar Omega
POLARstar Omega: - Fluorescence polarization
- Fluorescence intensity
- Simultaneous dual emission fluorescence
- Time-resolved fluorescence – TR-FRET (opt.)
- Luminescence (opt.)
- Simultaneous dual emission luminescence (opt.)
- UV/VIS absorbance spectra
- AlphaScreen
®
/ AlphaLISA
®
(opt.)
SPECTROstar Omega: - UV/VIS absorbance spectra
- Upgradeable to FLUOstar / POLARstar Omega
Light source
FLUOstar, POLARstar & SPECTROstar Omega: High-energy xenon flash-lamp
Detector - Side window photomultiplier tube
Filters
FLUOstar & POLARstar Omega: - 2 filter wheels with 8 excitation and 8 emission filter positions
LUMIstar Omega: - Emission filter wheel with 8 filter positions
Gain control - Software selectable gain
- Automatic gain adjustment
Plate carrier - Auto lock microplate carrier
- All microplate formats up to 1536-well -
Microplates should fulfill the SBS specification and non-SBS formats
should fit: (l
x
w
x
h) (mm) max: 128
x
86
x
22; min. length 124
Reagent Injection - Up to two built-in reagent injectors
- Individual injection volumes for each well
- Injection volumes definable down to 3µl
- Up to 4 independent injection actions per well
- Variable injection speed (100 µl/s to 420 µl/s)
Shaking - Linear, orbital and double-orbital shaking
- User-definable time and speed
Incubation - Incubation range from ambient +4°C to 45°C, in 0.1°C steps
- Extended incubation up to 65°C (optional)
- Temperature monitoring (without incubation)
- Temperature stability 0.2°C
Fluorescence intensity - Limit of detection 0.2 fmol / well fluorescein
- Spectral range (ex. and em.): 240 ... 740 nm (900 nm opt.)
- Dynamic range 8 decades
Time-resolved fluorescence - Limit of detection 30 amol / well europium
- Advanced TRF/TR-FRET: 3 amol / well europium
- Spectral range (ex. and em.): 240 ... 740 nm (900 nm opt.)
- Dynamic range: 6 decades