BMG LABTECH Omega Operating Manual
2014-05-16 0415B0001G 19/29
5.2.6 Absorbance Optics
This optic can only be used with readers with PMT based absorbance, not with
spectrometer based absorbance.
There is an optimized optic for absorbance mode available (see figure 15). This
optimized absorbance optic can be used for plate formats up to 384-well. The light
guide connects to the red-marked excitation-positioning wheel. This optic does not allow
for the use of injectors.
You can also use the combination optic (see chapter 5.2.5).
Figure 15:
Optimized
absorbance optic
5.2.7 High Density Optics
The high-density optic has one excitation light guide surrounded by six emission light
guides. This optic is designed to minimize cross-talk in plate formats such as 384-
and 1536-well plates.
To position the measurement head with the quick-fix holders, see chapter 5.2
Installation and Changing of Optics.
The single black light guide connects to the yellow-marked position of the excitation
wheel. The six-bundled light guides are inserted into the blue-marked position of the
emission wheel.
To measure 1536-well plates the reader has to be prepared specially.
Injection is not possible into 1536 well plates.
Figure 16: High
density optic
5.2.8 Bottom Optics
The bottom optics are used to measure fluorescence, luminescence and absorbance. The bottom optics
enter the reagent box on the left side and are connected to the left position of the excitation and emission
wheels.
Figure 17: Principle drawing of top
and bottom optics
To Next Page
Loading...
