Cellink INKREDIBLE - Bioprinting Process

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5.2 Bioprinng with the INKREDIBLE 3D
Bioprinter
The following steps should be followed every me you use your INKREDIBLE 3D
Bioprinter:
5.2.1 Installing the Cartridges
1. Aach nozzles to your cartridges with CELLINK Start (or other bioink of your
choosing).
2. Connect the pressure connectors for printheads [10a] and/or [10b] to the
printheads [2a] and/or [2b] by twisng the cartridge connectors.
3. Insert the cartridges inside the printheads [2a] [2b].
CAUTION! Verify there are two power outlets running on dierent fuses
available at the workspace, otherwise the air compressor might cause
electromagnec interference with the INKREDIBLE.
CAUTION! Press the cartridges down thoroughly prior to iniang the next
sequence.
4. Next, the INKREDIBLE 3D Bioprinter needs to be homed and calibrated.
5.2.2 Homing the Bioprinter:
1. Start by turning the INKREDIBLE on with the power buon [1].
2. Use the following sequense to home the INKREDIBLE 3D Bioprinter:
3. Remove all objects from the printbed [4] (such as well plates, petri
dishes etc.)
4. Lower the printbed [4] manually by simply pushing down at the back of the printbed.
5. Press the control knob [8] once to enter the menu.
6. Turn the control knob [8] to select “Prepare Bioprint” and press to enter.
7. Turn control knob [8] to “Home Axes” and press it to select. The INKREDIBLE
3D bioprinter will now home all axes. The nozzle of printhead 1 should
be in the middle of the printbed once the sequence is completed.
5.2.3 Calibrang the Z axis:
1. Next, the Z Axis needs to be adjusted and calibrated to bioprint in a petri dish. Use the
following sequence to do this:
2. Place a petri dish onto the printbed [4].
3. Press the control knob [8] once to enter the “Menu”.
straight out from the side of the INKREDIBLE 3D bioprinter.
WARNING! Never reach into the instrument while it is moving. Wait unl all
parts have come to a complete stop before reaching into the instrument.