Cellink INKREDIBLE - Page 24

24
4. Turn the control knob [8] to select “Prepare Bioprint” and press the control knob [8]
to enter.
5. Turn the control knob [8] to select “Move Z” and press the control knob to enter.
You have now entered Manual Z Calibraon Mode. On the display you should see:
Z:+040.0 (posion of Z axis: 40 mm).
6. Turn the control knob [8] unl the nozzle of printhead 1 is right above the petri dish. A
space of about 0.1 mm should be visible between the printbed and the p of the nozzle.
You can use a piece of paper to slide between the nozzle and the petri dish to measure
the distance. The paper should slide freely without too much fricon.
7. Once the correct distance has been selected, Press the control knob [8] once to exit
the Manual Calibraon Z mode.
8. Select “Calibrate Z” using the control knob, press the control knob to conrm.
5.2.4 Seng the pressure:
Finally the prinng pressure needs to be adjusted to ensure a good ow. To do this, use
the following sequence:
1. Unlock the pressure regulators [11a] and [11b] by pulling the knobs straight out from
the side of the INKREDIBLE 3D bioprinter.
2. Turn down the pressure in both printheads to 0 by turning the pressure regulators
[11a] and [11b] counter clockwise unl they reach a complete stop. The digital pressure
monitors, [5a] and [5b], should now display 0 kPA (If not, see “8.2.10 Pressure monitor
not displaying correct pressure”).
3. Turn on the compressor.
4. Turn the control knob [8] to select “Prepare Bioprint” and press to enter.
5. Turn the control knob [8] to select “Turn ON PH1”. Printhead 1 [2a] is now turned on.
CAUTION! When the pressure and the printhead are turned on the printhead
will immediately start dispensing prinng materal. Be sure to collect the spare
material in a separate container (for example a spare petri dish or lid) to avoid
it from interfering with the bioprint.
6. Adjust the air pressure to your desired ow by turning the pressure
regulator for printhead 1 [11a] (for CELLINK Start and a red nozzle, 28 kPa gives a good
ow of printmass).
7. When you have aquired your desired ow, press the control knob to turn o the
printhead (the printhead will turn on automacally when a bioprint is iniated).
8. Repeat this process for printhead 2 if you wish to print with dual print heads.