Celeris User Manual
14770-A || ECN # 844 || ECN Date: 28 July 2017 Page 7 of 23
Instructions for Use
Setting up for a test
Animal Preparation
This should be done before running a test.
Note: this protocol should be performed in accordance with your institution’s Animal Care and Use Committee
regulations, and may be subject to institutional approval.
If any part of the testing will be done in Scotopic (dark-adapted) conditions, the animal should be left in a light-
proof dark room overnight. Be sure to review your institution’s policy regarding overnight housing in advance.
1. Anesthetize the animal. The most common anesthetic for rodent electrophysiology is an intraperitoneal
(IP) injection of ketamine and xylazine, as some gas anesthetics have been found to affect the ERG
response. When using the ketamine-xylazine cocktail, it is important to weigh the animal first so as to
calculate the correct dose. Improper dosing can lead to the death of the animal, or the animal failing to
become fully anesthetized.
2. Maximally dilate the animal’s pupil. Typically a combination of Phenylephrine HCl and either
Tropicamide or Atropine is used. Allow the drops to sit on the eyes for approximately 2 minutes before
wicking excess fluid from the eye in order to prevent inadvertent aspiration. Hydrating drops should
now be applied to the eye to prevent corneal drying and subsequent cataracts. A .3% hypromellose
lubricating gel solution is best; saline or artificial tears will need frequent reapplication to keep the eye
hydrated.
3. Optional – you may wish to trim the whiskers. This is not necessary but may make it easier to place
corneal electrodes, particularly the pattern stimulator electrode.
Environmental Preparation
Cover or turn off any light sources in the room. Light sources covered with red filters may be used but may affect
dark adaptation, due to the wide passband of many filters. The room should be darkened. Ensure that the Celeris
is turned on and that the heating pad has been permitted to come up to temperature. A recovery area for
animals recovering from anesthesia should be prepared in advance.
Software Preparation
From the launch page of the software, select the correct Examiner from the dropdown (for further information
on adding or modifying examiner names to the software, see Local Configuration Settings) and press the Start
button.
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