DLab D2012 plus - Page 21

USER MANUAL
18
Sterilization
Autoclaving
115℃（0.7kg/cm
2
）30minutes
O
O
O
121℃（1.0kg/cm
2
）20 minutes
X
O
O
126℃（1.4kg/cm
2
）15 minutes
X
X
X
Boiling
15 to 30 minutes
O
O
O
Ultraviolet sterilization
200-300nm
X
X
X
Gas sterilization
Ethylene oxide
O
X
O
Formaldehyde
O
O
O
PA: Polyallomer；PC：Polycarbonate；PP：Polypropylene
11.2.2 Cleaning PC tubes
PC materials are low in chemical resistance against alkaline solutions. Avoid using neutral detergents with
pH higher than 9. Note that pH of some neutral detergents are still higher than 9 even if diluted according to
the instruction in the maker’s catalog. Use detergent with its pH between 7 and 9.
11.2.3 Autoclaving PA、PC and PP tubes
PA begins softening at about 120℃, PC and PP at about 130℃. Autoclave PA tubes at 115℃（ 0.7kg/cm
2
）for
30 minutes and PC and PP tubes at 121℃（0.1kg/cm
2
）for 20 minutes. If a certain temperature is exceeded,
the tubes may be deformed.
When using a sterilizing chamber, please operate as follows:
（1） Place tubes in vertical position, mouths upward. If tubes are placed sideways, they may deform
into an oval shape due to gravity.
（2） Remove screw nuts and inner covers to prevent from deformation or rupture.
（3） Wait until the sterilizing chamber cools down to the room temperature before the tubes are
removed.
11.2.4 Condition and life expectancy of tubes
The life expectancy of plastic tubes depends on the characteristics of samples, speed of the rotor used, and
temperature applied, and so on. When the plastic tubes are used for centrifuge of ordinary aqueous samples
(pH between 5 and 9), their life expectancies are defined as follows.
Be operated at the maximum speed:
High quality tubes (PA、PC、PP): 30-50 operations
Ordinary tubes(PA、PC、PP): around 10 operations（Using in low speed can extend the tube life）.
Life expectancy of tubes also depends on the pretreatment conditions such as cleaning and sterilization,
lifetime can be cut down.
Notice: Do not use damaged or cracked tubes.