Formulatrix NT8 - Sitting Drop Experiments

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NT8 User's Guide | Chapter 9: Sitting Drop Experiments
To use this template:
1. Click the Open Task List button .
2. Open the Sample folder and select the Sitting Drop MRC2 100 nL Protein and 100
nL Well Solution task list.
3. Click Open.
4. Set the protein volume by right-clicking the protein block image and selecting Set
Protein Volume. Select the column that you will pipette protein into and enter the
volume in the appropriate field (volume must be the same in each well in a column).
5. Optional: If you need to change the aspirate or dispense volumes, double-click the
task in the Task List and edit the volume.
6. Run your experiment by clicking the Run Task List button .
Option 2: Manually Set Up the NT8 Software
1. Mimic the NT8’s physical setup with the software:
● Select each microplate you stationed on the NT8 from the Plate Type Library, and
drag and drop it into the appropriate location in the software.
● Drag and drop the LV Tip Caddy from the Plate Type Library to the LV Tip Caddy
station.
● Right-click the protein block image and select Set Protein Volume. Type in the
volume of protein available in each well of each column in the tray that you plan
to use.
2. Add the Pick Tip command to the Task List by double-clicking the LV Tip Caddy
image.
3. Click the Repeat button on the Task List to mark the beginning of tasks you
will repeat on each column of your plate.
4. Add the Wash Tip command to the Task List by double-clicking the Wash Station
image.
5. Now you’ll begin adding aspirate and dispense commands. First, tell the NT8 to
aspirate from your protein source by adding an Aspirate command.
● Double-click the Protein Block image.
● Next to Action, select Aspirate. Next to Column, select the column you want to
aspirate the protein from. Next to Volume, type in the volume you want to
aspirate.
● Click OK.
6. Now you’ll tell the NT8 to aspirate your well solution.
● Double-click the microplate image to open the Task Details dialog box.
Repeat