Formulatrix NT8 - Hanging Drop Experiments

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NT8 User's Guide | Chapter 10: Hanging Drop Experiments
Important: The angled corners of the plate seal should be at the right side of the reference
plate. After the dispense, you will flip the reference plate horizontally to the right to place the
plate seal on the experiment plate.
● Attach the UPV Hanging Drop MRC Plate Seal to the reference plate:
q Tear off the tab at the angled side of the plate seal. Then, carefully remove the
liner from the entire seal.
q Dab a small amount of 70% Isopropanol on the reference plate. This helps adhere
the plate seal to the reference plate during the dispense.
q Using the crosshairs to align the plate seal to the reference plate, fasten the UVP
Hanging Drop MRC Plate Seal to the microplate.
● Place the reference plate on a microplate station (angled corners of plate seal should
be at the right).
● Place the protein strip on the protein block, and put the protein strip clamp on top of
the protein strip to hold it in place. You will pipette your protein into the protein block
after you set up the software.
Option 1: Set Up the NT8 Software Using the Hanging Drop Templates
A hanging drop experiment template is preinstalled with your software. You may use it if you
plan to combine 100 nL protein with 100 nL well solution for a 200 nL dispense. You must also
use the MRC 2 Well Crystallization Plate and the UVP Hanging Drop Plate Seal.
To use this template:
1. Click the Open Task List button .
2. Open the Sample folder and select the Hanging Drop MRC2 100 nL Protein And
100 nL Well Solution task list.
3. Click Open.
Option 2: Manually Set Up the NT8 Software
1. Mimic the NT8’s physical setup with the software:
● Select the MRC 2 Well Crystallization Plate from the Plate Type Library and drag
it onto the associated plate station in the software.
● Select the UVP Hanging Drop Plate Seal from the Plate Type Library and drag it
onto the associate plate station in the software.
● Drag the LV Tip Caddy from the Plate Type Library to the LV Tip Caddy station in
the software.