Formulatrix NT8 - LCP Experiments; Setting Up NT8 Hardware for LCP Dispense

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Given the nature of an LCP experiment, the set up for an LCP dispense requires more hardware
setup than other experiments. This tutorial walks you through hardware setup, provides two
options for setting up the dispense in the software, running the LCP dispense, and finally
attaching the LCP coverslip to the LCP base plate.
Set Up the NT8 Hardware
1. Prepare the LCP syringe (for help with using the LCP mixer, see Mixing LCP Drops).
2. Place the LV tip caddy in its station and the MRC 2 Well Crystallization Plate on
microplate station 2. Each well of the MRC 2 plate should contain about 50 µl.
3. Prepare the LCP Plate:
● Spray the LCP plate with a duster to remove any contaminants.
● Peel off the brown packing tape.
● Apply 2-3 drops of 70% Isopropanol to the reference plate surface.
● Aligning the four corners drop locations to the crosshairs on the reference plate
and affix the LCP plate to the reference plate.
4. Attach the LCP syringe to the NT8’s head.
● Using your left hand, align the syringe with the syringe bracket and hold it in
place.
● Using your right hand, move the joystick to align the plunger lock on the NT8
with the top of the plunger.
● Using your left hand, place the top of the plunger into the plunger lock, and
gently press the barrel of the syringe into the syringe bracket until it snaps into
place.