Gilson pipetman - 9 GUIDELINES FOR GOOD PIPETTING; Aspirate and dispense

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1) FitnewPIPETMANTipforthebestresults(seeChapter18).
For single channel models, push the tip-holder into the
tipusing a slighttwisting motionto ensurea rmand
airtight seal.
For multichannel models,PIPETMANTipsarebesttted
from the patented ROCKY RACK available only in our
TIPACKs and TOWERPACKs. ROCKY RACK is the dome-
shaped part of the pack that contains the tips. ROCKY
RACKmakesiteasytosecurelytthetipstoamultichannel
pipette, ensuring an airtight seal on all channels without
the need to use undue pressure or to touch the tips.
2) Pre-rinsethetip.Pre-rinsingconsistsofaspiratingtherst
volume of liquid and then dispensing it back into the same
vessel (or to waste). Subsequent volumes that you pipette
willhavelevelsofaccuracyandprecisionwithinspecica-
tions. Some liquids (e.g. protein-containing solutions and
organicsolvents)canleavealmofliquidontheinsidethe
9 GUIDELINES FOR GOOD PIPETTING
Aspirate and dispense
• They form an air-tight seal with the tip-holder,
preventing the leaks that cause poor accuracy and
a lack of precision.
• PIPETMANTIPS (except lter tips) may be auto-
clavedat121°Cfor20minutesat0.1MPa.
Do not autoclave PIPETMAN Filter Tips, they
have been sterilized!
To ensure the best performance from
your Gilson pipette, you shoud ALWAYS
use PIPETMAN Tips (in accordance with
ISO8655) system, because PIPETMAN Tips
were used to establish the specifications.
PIPETMAN Tips to use for best results
Pipette model Tips model Range Color code
P10M D10* 0.1 – 10 L Red
DL10 0.1 – 20 L
DF10 0.1 – 10 L
DFL10 0.1 – 10 L
P20M D200 2 – 200 L Pale yellow
DF30 2 – 30 L
P200M D200 2 – 200 L Yellow
DF200 2 – 200 L
P1000M D1000 100 – 1000 L Blue
D1200 100 – 1200 L
DF1000 100 – 1000 L
DF1200 100 – 1200 L
P8x20M, P12x20M DL10 0.1 – 20 L Pale yellow
DFL10 0.1 – 10 L
DF30 2 – 30 L
D200 2 – 200 L
P8x200M, P12x200M D200 2 – 200 L Yellow
D300 20 – 300 L
DF200 20 – 200 L
DF300 20 – 300 L
P8x300M, P12x300M D300 20 – 300 L Green
DF300 20 – 300 L
* A plastic adapter is required to eject D10 and DF10
tips (short tips). The adapter is supplied with P10M.
No adapter is required when using DL10 and DFL10
tips (long tips).