Gilson pipetman - General Guidelines for Good Pipetting

ENGLISH
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wallofthetip;pre-rinsingthetipminimizesanyerrorsthatmayberelatedtothisphenomenon.
3) Holdthepipetteverticallyandimmersethetipintheliquid(seepage13).Pressthepush-button
toaspiratethesetvolumeofliquid.Waitafewseconds(timedependsonmodel,seepage13);
then withdraw the pipette-tip from the liquid. You may wipe any droplets away from the outside
of the tip using a medical wipe, however if you do so takecaretoavoidtouchingthetip’sorice.
4) Placetheendofthetipagainsttheinsidewalloftherecipientvessel(atanangleof10°to40°).
Pressthepush-button.Waitforatleastafewsecondsbeforereleasingthepush-buttontoexpel
anyresidualliquidfromthetip.Whileremovingthepipettedrawthetipalongtheinsidesurface
of the vessel.
5) Eject thetipbypressingrmlyonthetipejectorbutton.
General Guidelines for Good Pipetting
a.Makesurethatyoutnewtips.
b. Each new tip should be pre-rinsed with the liquid to be pipetted.
c.Whenaspirating,keepthetipataconstantdepthbelowthe
surface of the liquid (refer to Table).
d. Change the tip before aspirating a dierent liquid, sample, or
reagent.
e. Change the tip if a droplet remains at the end of the tip from
the previous pipetting operation.
f. Liquid should never enter the tip holder. To prevent this:
 •Neverturnthepipetteupsidedown,
•Neverlaythepipetteonitssidewhenthereisliquidinthetip(s),
g.TheGilsonStandAdapter(seeChapter2)isrecommendedforusewiththeCARROUSEL and SINGLE
PipetteHoldertostoreyourPIPETMANMpipetteintheverticalposition.AlternativelythePOWER
CARROUSELcanbeusedtostoreandchargeupto5PIPETMANMpipettes.
h.Whenpipettingliquidswithtemperaturesdierenttotheambienttemperature,pre-rinsethetip
several times before use in order to reach equilibrium between the temperatures of the liquid and
the pipette’s dead-volume.
i. For volatile liquids you should saturate the dead-volume by aspirating and dispensing the liquid
repeatedly before aspirating the sample.
j. After pipetting acids or other corrosive liquids that emit vapors, clean the pipette, as described in
Chapter 15.
k.Thepipettecanbeusedbetween+4°Cand+40°C,butthe
specicationsmayvary(seeChapter18).
l. Donotpipetteliquidshavingtemperaturesabove50°Cor
below4°C.
Immersion Depth and Wait Time
Model Immersion Depth Wait Time
(mm) (seconds)
P10M 1 1
P20M 2-3 1
P200M 2-4 1
P1000M 2-4 2-3
P8x20M, P12x20M 2-3 1
P8x200M, P12x200M 2-3 1
P8x300M, P12x300M 2-3 1
Extreme temperatures
can affect accuracy and
precision!