Absorbance and transmittance measurement procedure
Note: To measure samples with negative absorbance, set the analytical zero using the sample with the greatest absorbance, and measure the sample with the least
absorbance. Report the reading as negative absorbance.
1. Push UNITS/Exit
until "%T" or "ABS" is
shown on the display.
2. Using the manual
flow cell kit, install the
flow cell. Refer to Using
a flow cell
on page
31.
Note: The sample cell
cover does not close
when the flow cell is
installed.
3. Slowly put 250 mL of
100 %T or zero
absorbance reference
solution down the
interior edge of the inlet
reservoir.
Put the sample down
the interior edge of the
reservoir to prevent air
bubbles in the sample.
4. Push CAL/Zero.
The display shows "100
%T" or zero.
Note:
The instrument starts
analytical zeroing for
transmittance, and
absorbance modes at
the same time.
5. Push ENTER.
The instrument display
counts down from 30 to
0.
Note: If the value
shown is not 100 %T,
0.000 A, or if dashes
flash, do steps 5 and 6
again.
6. Slowly put 250 mL of
the sample in the inlet
reservoir.
28 English