Integra DOSE IT - Page 17

DOSE IT – Operating instructions V08 Operation
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4.3.4 Defining a customized program
The following example of a customized program can be used to fill 10 density gradient
tubes. The careful and continuous dispensing without pauses prevents stirring up of
layers.
Parameter Value Description of settings
Name Density 
gradient
Allows to name your customized program.
Tubing-ID 3.0 mm Define the inner diameter of the tubing inserted.
Dispense
Direction
 / C W  Define the rotational direction of the rotor, e. g. clock-
wise.
No. of heads 1 Define the number of pump heads installed.
Step 1: Dispense Volume: 2.5 ml
Repetitions: 1
Pause: manual (here without pause)
Flow rate: 3.8 ml/min (i.e. speed: 4.0 rpm).
Step 2: Dispense
Ramp
Volume: 12.5 ml
Repetitions: 1
Pause: manual (here without pause)
Start flow: 3.8 ml/min (i.e. speed: 4.0 rpm)
End flow: 22.8 ml/min (i.e. speed: 24.0 rpm).
Step 3: Wait Manual, i.e. the next cycle starting with step 1 has to be
activated manually by pressing N
EXT STEP.
Step 4: Cycles Number of cycles: 10
Goto step: 1.
The figure depicts the 
customized program 
described above.