Invitrogen Qubit 4 - Guidelines for Using the Qubit 4 Fluorometer; Assay Best Practices

16 Qubit
™
4 Fluorometer User Guide
Guidelines for using the Qubit
™
4 Fluorometer
Recommendations
To obtain the best results, follow the recommendations below. For more
information, see “Critical Qubit
™
Assay considerations”, page 57.
• Do not operate the instrument in direct sunlight.
• Wear gloves during sample handling.
• Use the instrument at room temperature only (22–28ºC).
• Bring all kit reagents to room temperature and insert all assay tubes into the
instrument only for as much time as it takes for the instrument to measure the
fluorescence.
• Do not hold the assay tubes in your hand before performing a measurement.
• Make sure that you have calibrated the Qubit
™
4 Fluorometer using the
appropriate standards.
• Incubate the tubes for the Qubit
™
DNA and RNA assays for 2 minutes after
mixing the sample or standard with the working solution.
• Incubate the tubes for the Qubit
™
protein assays for 15 minutes after mixing
the sample or standard with the working solution.
• If you are performing multiple readings of a single tube, remove the tube
from the instrument and let it equilibrate to room temperature for 30 seconds
before taking another reading.
Note: Multiple readings of RNA samples is not recommended.
• Visit thermofisher.com/qubit
for additional application notes, technical notes,
citations, software updates, and a list of validated Qubit
™
assays that have
been tested using the Qubit
™
4 Fluorometer.
Assay tubes for the
Qubit
™
4
Fluorometer
Only thin-wall, clear 0.5-mL PCR tubes are appropriate for use in the Qubit
™
4
Fluorometer. Acceptable tubes include Qubit
™
assay tubes (Cat. No. Q32856,
500 tubes). The minimum assay volume must be 200 µL for an accurate read.